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美利体育登录入口官网:DIN EN ISO 9936:2011
动植物油脂.通过高性能液相色谱法测定生育酚和生育三烯酚含量(ISO 9936-2006 + Amd.1-2011);德文版本EN ISO 9936-2006 + A1-2011

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2006 + Amd.1:2011); German version EN ISO 9936:2006 + A1:2011


标准号
DIN EN ISO 9936:2011
发布
2011年
国际标准分类(ICS)
67.200.10 动物和植物的脂肪和油   
总页数
24页
发布单位
德国标准化学会
替代标准
DIN EN ISO 9936:2016
DIN EN ISO 9936:2011-11
当前最新
DIN EN ISO 9936:2016-11
 
 
引用标准
ISO 661
被代替标准
DIN EN ISO 9936:2006 DIN EN ISO 9936/A1:2010
适用范围
本标准规定了动植物油脂(简称脂肪)中游离α-、β-、γ-、γ-生育酚和生育三烯酚(统称母育酚)含量的测定方法。通过高效液相色谱(HPLC)。
术语描述
Tocolgehalt
Tocol content
Mass fractions of individual Tocols determined by the procedure specified in this International Standard. The content is expressed in milligrams per kilogram as a whole number.
Wiederholpr?zision
Repeatability precision
The closeness of agreement between independent test results obtained under repeatability conditions (same laboratory, same operator, same equipment, short interval of time). Expressed as the absolute difference not exceeded in not more than 5% of cases.
Vergleichpr?zision
Reproducibility precision
The closeness of agreement between independent test results obtained under reproducibility conditions (different laboratories, different operators, different equipment). Expressed as the absolute difference not exceeded in not more than 5% of cases.
Verseifung
Saponification
A pre-treatment procedure required for products containing esters of tocopherols or tocotrienols before chromatography to determine the total free content.
Kalibrierung
Calibration
The process of establishing the relationship between the response of the measuring instrument (peak area) and the concentration of the analyte using standard solutions.

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HPLC-Analysens?ule

Typ 1: 250 mm x 4 mm, Diol-Kieselgel (5 ?m) Typ 2: 250 mm x 4,6 mm, Kieselgel (5 ?m)
Column used for chromatographic separation of Tocols.
HPLC-System

Bestehend aus Hochdruckpumpe, Probeneinspritzger?t, Thermostat (25 °C), Fluoreszenzdetektor (Anregung 295 nm, Emission 330 nm) oder UV-Detektor (292 nm)
Used for the separation and detection of Tocopherols and Tocotrienols.
Rotationsverdampfer

Nennvolumen geeignet, Temperatur nicht über 40 °C
Used to evaporate solvents under vacuum.
UV-Spektrometer

Mit Küvettenl?nge 10 mm, Messbereich 270-310 nm
Used to measure the extinction of standard solutions for concentration determination.

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